【关键词】 盐酸塔斯品碱
［摘要］目的：研究盐酸塔斯品碱对皮肤创伤愈合的促进作用及其机制。方法：建立大鼠背部双侧圆形创伤模型，观察创伤创面的愈合时间，测量其面积，检测组织中羟脯氨酸与蛋白质含量的变化，并进行组织形态学观察。结果：盐酸塔斯品碱可明显缩短创面的愈合时间。创伤后第3～14天，盐酸塔斯品碱组大鼠创面愈合率显著高于自身二甲亚砜对照组（P<0.05或P<0.01）；创伤后第3～7天，大鼠创伤肉芽组织中蛋白质含量在盐酸塔斯品碱2 mg/ml组明显高于模型组（P<0.05），创伤后第7天蛋白质含量达到峰值，第14～21天逐渐降至正常皮肤组织的含量水平；创伤后第3～21天，大鼠创伤组织中羟脯氨酸的含量，在盐酸塔斯品碱组明显高于模型组（P<0.05或P<0.01），创伤后第14天羟脯氨酸的含量达到峰值，第21天接近正常皮肤组织的含量水平。组织形态学结果显示：在大鼠创伤组织修复早期，盐酸塔斯品碱能够促进新生毛细血管的形成。结论：盐酸塔斯品碱具有促进大鼠皮肤创伤愈合的作用，其作用机制可能与促进肉芽组织中新生毛细血管的生成以及蛋白质和胶原的合成有关。
［关键词］盐酸塔斯品碱; 伤口愈合; 蛋白质类; 羟脯氨酸; 毛细血管
Effect of taspine hydrochloride on skin wound healing in rats and its mechanism
ABSTRACT Objective: To study the effect of taspine hydrochloride (TA/HCl) on skin wound healing in rats and its mechanism. Methods: Bilateral round wounds were made on the backs of SD rats. The effect of TA/HCl on the skin wound was evaluated through determining closure time and contracting ability of the skin wound, observing histopathological characteristics and measuring contents of hydroxyproline (Hyp) and protein in the wound tissue. Results: The closure time of the skin wounds was significantly shorter in the TA/HCltreated groups than that in the model group. The percentages of wound contraction were higher in the TA/HCltreated groups than that in the dimethyl sulfoxide (DMSO) control group of the same group(P<0.05 or P<0.01) on the 3rd to 14th days after wounding. The content of the protein in the wound tissue in the TA/HCltreated group (2 mg/ml ) was higher than that in the model group (P<0.05) on the 3rd to 7th days after wounding, and it arrived at the peak on the 7th day and gradually decreased to the normal level in skin tissue on the 14th to 21st days after wounding. The contents of Hyp in the wound tissues in the TA/HCltreated groups were higher than that in the model group (P<0.05 or P<0.015) on the 3rd to 21st days after wounding, and they arrived at the peak on the 14th day and at the normal level in skin tissue on the 21st day. Histopathological test results showed that TA/HCl could promote the formation of newly born capillaries in the early period of the wound healing. Conclusion: TA/HCl has the ability of promoting skin wound healing in rats, and it can also accelerate the growth of newly born capillaries and raise the production of protein and collagen in wound tissue.
KEY WORDS taspine hydrochloride; wound healing; proteins; hydroxyproline; capillaries
红毛七［Leontice robustum (Maxim.) Diels］为小檗科牡丹草属植物类叶牡丹的根茎，是陕西秦巴山区特有的药用植物，民间用于治疗关节炎和跌打损伤等［1］。2003年高琨等［2］首次用细胞膜色谱法筛选研究了红毛七有效成分，发现红毛七中含有的塔斯品碱（taspine）等多种生物碱与血管内皮细胞膜及成纤维细胞膜有很强的亲合性。国外文献报道，从南美植物巴豆属Croton lechleri中提取的塔斯品碱具有抗炎和促进伤口愈合的作用［3～5］，但其作用机制尚未完全阐明。本实验从红毛七中分离获得塔斯品碱并制备成盐酸塔斯品碱（taspine hydrochloride, TA/HCl），在筛选研究的基础上，以碱性成纤维细胞生长因子（basic fibroblast growth factor, bFGF）为对照，观察TA/HCl对大鼠皮肤创伤创面的愈合作用，并对其可能的作用机制进行了初步的探讨。现报道如下。
1材料与方法
1.1材料 （1）TA/HCl（西安交通大学天然药物研究与工程中心制 1 2 3 4 下一页
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