【关键词】 幽门螺杆菌
Immunzition treatment of Helicobacter pylori infection with two valence vaccine composed of catalase and adhesin conservation: An animal model study
【Abstract】 AIM: To investigate the effects of two valence vaccine consisting of Helicobacter pylori (H. pylori) catalase and adhesin conservation in the treatment of H. pylori infection in an H. pylori infected mouse model. METHODS: C57BL/6 mice infected with H. pylori were divided into seven groups. The mice of each group were immunized respectively by catalase and adhesin conservation (separately, 100 μg) plus CT (2 μg), catalase (100 μg) plus CT (2 μg), adhesin conservation (100 μg) plus CT (2 μg), catalase (100 μg), adhesin conservation (100 μg) and CT (2 μg), PBS orally once a week for four weeks. Four weeks after the last treatment, all animals were sacrificed and stomach biopsies were collected to detect H. pylori by semiquantitative bacterial culture assay. RESULTS: The eradication rate of two valence vaccine group, single valence vaccine group of catalase and single valence vaccine group of adhesin conservation was 69.2%（18/26）, 30.8%（8/26） and 38.5%(10/26), respectively. The eradication rate of the other four groups was all 0%. The H. pylori colonized density of the vaccination group was significantly lower than that of the other four groups (P<0.05). There were significantly differences in the eradication rate and H. pylori colonized density between the two valence vaccination group and the single valence vaccination groups (P<0.05). CONCLUSION: The two valence vaccine consisting of catalase, adhesion conservation and adjuvant has better immunotherapeutic effects than the single valence vaccine. More H. pylori vaccine research should be directed to the multivalence vaccine.
【Keywords】 Helicobacter pylori; catalase; adhesin; vaccine
【摘要】 目的： 利用人类幽门螺杆菌(H.pylori)感染的小鼠模型研究过氧化氢酶和黏附素保守区二价疫苗治疗H.pylori感染的作用. 方法： 把已感染H.pylori的小白鼠分成7组, 分别通过灌胃方法给予过氧化氢酶和黏附素保守区(各100 μg)加霍乱毒素CT(2 μg)、过氧化氢酶(100 μg)加CT (2 μg)、黏附素保守区(100 μg)加CT (2 μg)，PBS，单纯过氧化氢酶(100 μg)、单纯黏附素保守区(100 μg)、单纯CT (2 μg),1次/wk,共4次,治疗结束后4 wk处死动物,取胃黏膜行半定量细菌培养检查H.pylori情况. 结果： 治疗实验各组H.pylori根除率分别为: 过氧化氢酶和黏附素保守区加CT组69.2%（18/26）、过氧化氢酶加CT组30.8%（8/26）、黏附素保守区加CT组38.5%(10/26)；生理盐水组、单纯过氧化氢酶、单纯黏附素保守区、单纯CT组根除率均为0%. 未根除H.pylori的小鼠，疫苗治疗组H.pylori的定植密度明显低于其他4组（P<0.05）. 此外，二价疫苗组的H.pylori根除率及定植密度较单价疫苗组均有显著性差异（P<0.05）. 结论： 由过氧化氢酶和黏附素保守区加免疫佐剂组成的二价口服疫苗较单价口服疫苗有更好的免疫治疗效果,多价疫苗是未来H.pylori疫苗的研制方向.
【关键词】 幽门螺杆菌；过氧化氢酶；黏附素；疫苗
0引言
我们已构建了幽门螺杆菌（Helicobacter pylori, H.pylori）黏附素保守区AB和过氧化氢酶的高效原核表达体系［1,2］，表达产物经过纯化后在体外探讨了它的安全性、免疫原性和生物活性［3］. 现通过建立感染H.pylori的小鼠模型观察过氧化氢酶和黏附素保守区二价疫苗治疗H.pylori感染的作用，为治疗性H.pylori疫苗的研制奠定基础.
1材料和方法
1.1材料
幽门螺杆菌ss1株为本所保存，无特定病原体（specificpathogen free， SPF）C57BL/6小鼠140只，4 wk龄，雄性，体质量12~13 g，饲养条件符合2级动物要求. 重组黏附素保守区rAB、过氧化氢酶、快速尿素酶试剂由本所制备；其余试剂为国产分析纯. H.pylori的固体培养：取冷冻保存的H.pylori菌种一环于固体培养基上连续划线接种，2 h内置厌氧培养箱，37℃微需氧环境（5 1 2 3 下一页
本文版权归原作者所有，如需转载或摘录请注明出处:论文网
无相关信息